Crystallographic studies of P-type ATPase cation pumps
نویسندگان
چکیده
منابع مشابه
Structures of P-type and F-type ion pumps
P-type ion pumps are large, integral membrane proteins, whereas the F-type ATP synthase is an enormous multiple-subunit complex. Both have been the focal points of intensive physiological and mechanistic studies for many years; however, critical information about three-dimensional structure has been lacking. During the past year, new crystal forms of both P-type and F-type ATPases were reported...
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In the presence of ascorbate/H2O2, Fe 2 ions or the ATP-Fe complex catalyze selective cleavage of the subunit of gastric H ,K -ATPase. The electrophoretic mobilities of the fragments and dependence of the cleavage patterns on E1 and E2 conformational states are essentially identical to those described previously for renal Na ,K -ATPase. The cleavage pattern of H ,K ATPase by Fe ions is consiste...
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In the presence of ascorbate/H(2)O(2), Fe(2+) ions or the ATP-Fe(2+) complex catalyze selective cleavage of the alpha subunit of gastric H(+),K(+)-ATPase. The electrophoretic mobilities of the fragments and dependence of the cleavage patterns on E(1) and E(2) conformational states are essentially identical to those described previously for renal Na(+),K(+)-ATPase. The cleavage pattern of H(+),K...
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The vacuolar H(+)-ATPases (V-ATPases) are a universal class of proton pumps that are structurally similar to the F-ATPases. Both protein families are characterized by a membrane-bound segment (V(o), F(o)) responsible for the translocation of protons, and a soluble portion, (V(1), F(1)), which supplies the energy for translocation by hydrolyzing ATP. Here we present a mechanochemical model for t...
متن کاملStructural studies of P-type ATPase–ligand complexes using an X-ray free-electron laser
Membrane proteins are key players in biological systems, mediating signalling events and the specific transport of e.g. ions and metabolites. Consequently, membrane proteins are targeted by a large number of currently approved drugs. Understanding their functions and molecular mechanisms is greatly dependent on structural information, not least on complexes with functionally or medically import...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2010
ISSN: 0108-7673
DOI: 10.1107/s010876731009985x